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Input:
Sequence Data File:
(format)
Note: Maximum of 30 sequences :: 1000 nucleotides
Parameters:
Monovalent Salt (Molar)
(Explain this)
Total Strand Concentration (µM)
(Explain this)
Temperature (ΔG calculations)(°C)
(Explain this)
Desired Tm Range for SNP Primers
Min:
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
Max:
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
Desired Size Range for SNP Primers
Min:
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Max:
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Number Primers to Return
2
3
4
5
6
7
8
Calculate ASPE Results
© 2005 National Institute of Standards and Technology
